How to Use the Equinox 55 Bruker Spectrometer: A Comprehensive Guide
The Equinox 55 Bruker Spectrometer is a Fourier transform infrared (FTIR) and near-infrared (FTNIR) spectrometer that can be used for various applications such as chemical analysis, material characterization, and spectroscopy. This guide will provide you with the basic steps to operate the spectrometer and obtain reliable results.
How to Use the Equinox 55 Bruker Spectrometer: A Comprehensive Guide
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What is FTIR and FTNIR Spectroscopy?
FTIR and FTNIR spectroscopy are techniques that use infrared radiation to measure the absorption or emission spectra of a sample. The spectra can reveal information about the molecular structure, composition, and interactions of the sample. FTIR and FTNIR spectroscopy are based on the principle that different molecular bonds vibrate at different frequencies when exposed to infrared radiation. By measuring the intensity of the radiation that is absorbed or emitted by the sample at different wavelengths, a spectrum can be obtained that reflects the molecular fingerprint of the sample.
What are the Components of the Equinox 55 Bruker Spectrometer?
The Equinox 55 Bruker Spectrometer consists of four main components: a source, an interferometer, a sample compartment, and a detector. A schematic diagram of the spectrometer is shown below[^4^].
The source is a device that emits infrared radiation with a broad range of wavelengths. The interferometer is a device that splits the radiation into two beams and recombines them after they have traveled different optical paths. The interference pattern of the recombined beams depends on the difference in optical path length between the two beams. By varying this difference with a moving mirror, a modulated signal can be obtained that contains information about all the wavelengths in the source. This signal is called an interferogram.
The sample compartment is where the sample is placed for analysis. Depending on the type and form of the sample, different accessories can be used to hold or prepare the sample. For example, solid samples can be pressed into pellets or mounted on windows, liquid samples can be placed in cells or cuvettes, powder samples can be mixed with KBr or Nujol, and gas samples can be flowed through tubes or cells. The sample compartment also has ports for connecting external devices such as temperature controllers or light-fiber probes.
The detector is a device that converts the modulated signal from the interferometer into an electrical signal that can be processed by a computer. The detector must be sensitive to the wavelength range of interest and have a fast response time. Different types of detectors are available for different applications. For example, mercury cadmium telluride (MCT) detectors are commonly used for mid-infrared (MIR) spectroscopy, while indium gallium arsenide (InGaAs) detectors are suitable for near-infrared (NIR) spectroscopy.
How to Operate the Equinox 55 Bruker Spectrometer?
The following steps describe how to operate the Equinox 55 Bruker Spectrometer using the OPUS software:
Turn on the spectrometer and wait for it to warm up. This may take up to 30 minutes.
Launch the OPUS software and select \"Measurement\" from the menu bar.
Select \"Setup\" from the drop-down menu and choose the appropriate parameters for your measurement. For example, you can select the spectral range, resolution, number of scans, apodization function, phase correction method, etc.
Select \"Background\" from the drop-down menu and place an empty sample holder or a reference material in the sample compartment. Click \"Start\" to record a background spectrum. This spectrum will be used to correct for any instrumental effects or environmental influences.
Select \"Sample\" from the drop-down menu and place your sample in the sample compartment. Click \"Start\" to record a sample spectrum. 04f6b60f66